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Quick Overview
DNA Target
Probe-Based Detecion Chemistry
ROX Included
Multiplex Capable
150 rxn
Probe-Based Detecion Chemistry
ROX Included
Multiplex Capable
150 rxn
Details
Takyon™ ROX Probe 5X MasterMix dTTP is a convenient, optimized 5X concentrated mixture of premeasured ingredients in a ready-to-use format for the performance of real-time PCR detection of universal DNA targets using fluorescent, gene-specific probe molecules. Obtain consistent and sensitive results under Fast or regular cycling conditions. 5X concentrated reaction buffer offers the advantage of more dead volume for DNA template. MasterMix is formulated with a chemically modified hot-start 3rd generation Phusion DNA polymerase that is activated in just three minutes at 95°C. Incorporates ROX passive reference dye to permit sample fluorescence normalization. Refer to Technical Data Sheet for kit components and cycling conditions. Compatible with ABI Prism® 7000, 7300, 7700 & 7900 and ABI StepOne™ & StepOnePlus™.
Additional Information
| Applications | qPCR |
|---|---|
| RT System Compatibility | N/A |
| Detection Chemistry | Probe |
| dUTP | No |
| Fast Protocol Compatible | Yes |
| Passive Reference Dye | ROX |
| Hot Start | Yes |
| Concentration | 5X |
| Multiplex Capable | Yes |
| Number of Reactions | 150 |
| Kit Volume | 0.6 ml |
| Reaction Volume (µL) | 20 µL |
| Target | DNA |
| UNG Included | No |
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